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Image Search Results
Journal: Nanomaterials
Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness
doi: 10.3390/nano10101951
Figure Lengend Snippet: The characterization of two kinds of iron nanoparticles and their effects on KG1a and HL60: ( A , B ) hydrated particle size and zeta potential of Prussian blue nanoparticles (PBNPs) ( A ) and Fe 3 O 4 nanoparticles (FeNPs) ( B ), ( C ) cell viability of the PBNP- and FeNP-treated HL60 and KG1a cells, ( D ) cellular iron contents of the PBNP- and FeNP-treated HL60 and KG1a cells, and ( E ) QPCR detection of CD34 and CD38 expression in two cells. HF, FeNP-treated HL60; HP, PBNP-treated HL60; KF, FeNP-treated KG1a; KP, PBNP-treated KG1a. ns, no significance; *, p < 0.05; **, p < 0.01; ***, p < 0.001. ( F ) ROS levels of the PBNP- and FeNP-treated HL60 and KG1a cells.
Article Snippet: The human
Techniques: Zeta Potential Analyzer, Expressing
Journal: Nanomaterials
Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness
doi: 10.3390/nano10101951
Figure Lengend Snippet: Comparisons of differentially expressed genes (DEGs) in two Leukemia cells treated with two kinds of iron nanoparticles: ( A ) number of upregulated and downregulated DEGs (fold change > 1.0) in the PBNP- and FeNP-treated HL60 and KG1a cells and their relationship, ( B ) common DEGs (fold change > 1.5) in a cell treated by two kinds of nanoparticles, ( C ) common DEGs (fold change > 1.5) in two cells treated by a nanoparticle, ( D ) common DEGs (fold change > 1.0) in two cells treated by two kinds of nanoparticles, and ( E ) schematic of cellular functions of common DEGs (fold change > 1.0) in two cells treated by two kinds of nanoparticles. The detailed information of all DEGs is shown in . HF, FeNP-treated HL60 cells; HP, PBNP-treated HL60 cells; KF, FeNP-treated KG1a cells; and KP, PBNP-treated KG1a cells.
Article Snippet: The human
Techniques:
Journal: Nanomaterials
Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness
doi: 10.3390/nano10101951
Figure Lengend Snippet: Top 20 GO terms of the KG1a cells treated with two kinds of iron nanoparticles: ( A , B ) heatmap ( A ) and enrichment network ( B ) colored by the same cluster of GO terms in the FeNP-treated KG1a cells, and ( C , D ) heatmap ( C ) and enrichment network ( D ) colored by the same cluster of GO terms in the PBNP-treated KG1a cells. The colored labels followed the order of top 20 GO terms. ( E ) Venn analysis of top 20 GO terms in the KG1a cells treated with PBNPs and FeNPs. The detailed information of all GO terms is shown in .
Article Snippet: The human
Techniques:
Journal: Nanomaterials
Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness
doi: 10.3390/nano10101951
Figure Lengend Snippet: GO terms of lipid metabolisms in KG1a and HL60 exposed to FeNPs or PBNPs (Top 20, p < 0.01).
Article Snippet: The human
Techniques: Membrane, Activity Assay
Journal: Nanomaterials
Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness
doi: 10.3390/nano10101951
Figure Lengend Snippet: GO terms of metal ion metabolisms in KG1a and HL60 exposed to FeNPs or PBNPs (Top 20, p < 0.01).
Article Snippet: The human
Techniques: Activity Assay, Clinical Proteomics, Membrane
Journal: Nanomaterials
Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness
doi: 10.3390/nano10101951
Figure Lengend Snippet: Comparative Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the DEGs ( p < 0.05): ( A ) comparative top 10 enriched pathways of HL60 and KG1a treated with FeNPs and ( B ) comparative top 10 enriched pathways of HL60 and KG1a treated with PBNPs. The pathways in black and blue were HL60 and KG1a cells, respectively. ( C ) Four-way Venn analysis of all KEGG pathways in four groups. The detailed information of all KEGG pathways is shown in . HF, FeNP-treated HL60 cells; HP, PBNP-treated HL60 cells; KF, FeNP-treated KG1a cells; and KP, PBNP-treated KG1a cells.
Article Snippet: The human
Techniques:
Journal: Nanomaterials
Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness
doi: 10.3390/nano10101951
Figure Lengend Snippet: Validation of RNA-Seq DEGs using RT-qPCR: ( A , B ) the expression levels of 10 selected DEGs in the iron nanoparticle-treated HL60 ( A ) and KG1a ( B ) cells. All values are mean ± SD with n = 3. ns, no significance; *, p < 0.05; **, p < 0.01, ***, p < 0.001, ****, and p < 0.0001. ( C , D ) The comparison of fold change detected by RT-qPCR and RNA-Seq in the iron nanoparticle-treated HL60 and ( C , D ) KG1a cells.
Article Snippet: The human
Techniques: Biomarker Discovery, RNA Sequencing, Quantitative RT-PCR, Expressing, Comparison