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China Center for Type Culture Collection human acute myelogenous leukemia (aml) cell kg1a
The characterization of two kinds of iron nanoparticles and their effects on <t>KG1a</t> and HL60: ( A , B ) hydrated particle size and zeta potential of Prussian blue nanoparticles (PBNPs) ( A ) and Fe 3 O 4 nanoparticles (FeNPs) ( B ), ( C ) cell viability of the PBNP- and FeNP-treated HL60 and KG1a cells, ( D ) cellular iron contents of the PBNP- and FeNP-treated HL60 and KG1a cells, and ( E ) QPCR detection of CD34 and CD38 expression in two cells. HF, FeNP-treated HL60; HP, PBNP-treated HL60; KF, FeNP-treated KG1a; KP, PBNP-treated KG1a. ns, no significance; *, p < 0.05; **, p < 0.01; ***, p < 0.001. ( F ) ROS levels of the PBNP- and FeNP-treated HL60 and KG1a cells.
Human Acute Myelogenous Leukemia (Aml) Cell Kg1a, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AllCells LLC acute myelogenous leukemia (aml) peripheral blood mononuclear cells
The characterization of two kinds of iron nanoparticles and their effects on <t>KG1a</t> and HL60: ( A , B ) hydrated particle size and zeta potential of Prussian blue nanoparticles (PBNPs) ( A ) and Fe 3 O 4 nanoparticles (FeNPs) ( B ), ( C ) cell viability of the PBNP- and FeNP-treated HL60 and KG1a cells, ( D ) cellular iron contents of the PBNP- and FeNP-treated HL60 and KG1a cells, and ( E ) QPCR detection of CD34 and CD38 expression in two cells. HF, FeNP-treated HL60; HP, PBNP-treated HL60; KF, FeNP-treated KG1a; KP, PBNP-treated KG1a. ns, no significance; *, p < 0.05; **, p < 0.01; ***, p < 0.001. ( F ) ROS levels of the PBNP- and FeNP-treated HL60 and KG1a cells.
Acute Myelogenous Leukemia (Aml) Peripheral Blood Mononuclear Cells, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ranbaxy Pharmaceuticals Canada acute myelogeneous leukemia (aml) with t(14;17) (q32; q11.2)
The characterization of two kinds of iron nanoparticles and their effects on <t>KG1a</t> and HL60: ( A , B ) hydrated particle size and zeta potential of Prussian blue nanoparticles (PBNPs) ( A ) and Fe 3 O 4 nanoparticles (FeNPs) ( B ), ( C ) cell viability of the PBNP- and FeNP-treated HL60 and KG1a cells, ( D ) cellular iron contents of the PBNP- and FeNP-treated HL60 and KG1a cells, and ( E ) QPCR detection of CD34 and CD38 expression in two cells. HF, FeNP-treated HL60; HP, PBNP-treated HL60; KF, FeNP-treated KG1a; KP, PBNP-treated KG1a. ns, no significance; *, p < 0.05; **, p < 0.01; ***, p < 0.001. ( F ) ROS levels of the PBNP- and FeNP-treated HL60 and KG1a cells.
Acute Myelogeneous Leukemia (Aml) With T(14;17) (Q32; Q11.2), supplied by Ranbaxy Pharmaceuticals Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mastocytosis Society acute myelogenous leukemia aml
The characterization of two kinds of iron nanoparticles and their effects on <t>KG1a</t> and HL60: ( A , B ) hydrated particle size and zeta potential of Prussian blue nanoparticles (PBNPs) ( A ) and Fe 3 O 4 nanoparticles (FeNPs) ( B ), ( C ) cell viability of the PBNP- and FeNP-treated HL60 and KG1a cells, ( D ) cellular iron contents of the PBNP- and FeNP-treated HL60 and KG1a cells, and ( E ) QPCR detection of CD34 and CD38 expression in two cells. HF, FeNP-treated HL60; HP, PBNP-treated HL60; KF, FeNP-treated KG1a; KP, PBNP-treated KG1a. ns, no significance; *, p < 0.05; **, p < 0.01; ***, p < 0.001. ( F ) ROS levels of the PBNP- and FeNP-treated HL60 and KG1a cells.
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The characterization of two kinds of iron nanoparticles and their effects on KG1a and HL60: ( A , B ) hydrated particle size and zeta potential of Prussian blue nanoparticles (PBNPs) ( A ) and Fe 3 O 4 nanoparticles (FeNPs) ( B ), ( C ) cell viability of the PBNP- and FeNP-treated HL60 and KG1a cells, ( D ) cellular iron contents of the PBNP- and FeNP-treated HL60 and KG1a cells, and ( E ) QPCR detection of CD34 and CD38 expression in two cells. HF, FeNP-treated HL60; HP, PBNP-treated HL60; KF, FeNP-treated KG1a; KP, PBNP-treated KG1a. ns, no significance; *, p < 0.05; **, p < 0.01; ***, p < 0.001. ( F ) ROS levels of the PBNP- and FeNP-treated HL60 and KG1a cells.

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: The characterization of two kinds of iron nanoparticles and their effects on KG1a and HL60: ( A , B ) hydrated particle size and zeta potential of Prussian blue nanoparticles (PBNPs) ( A ) and Fe 3 O 4 nanoparticles (FeNPs) ( B ), ( C ) cell viability of the PBNP- and FeNP-treated HL60 and KG1a cells, ( D ) cellular iron contents of the PBNP- and FeNP-treated HL60 and KG1a cells, and ( E ) QPCR detection of CD34 and CD38 expression in two cells. HF, FeNP-treated HL60; HP, PBNP-treated HL60; KF, FeNP-treated KG1a; KP, PBNP-treated KG1a. ns, no significance; *, p < 0.05; **, p < 0.01; ***, p < 0.001. ( F ) ROS levels of the PBNP- and FeNP-treated HL60 and KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Zeta Potential Analyzer, Expressing

Comparisons of differentially expressed genes (DEGs) in two Leukemia cells treated with two kinds of iron nanoparticles: ( A ) number of upregulated and downregulated DEGs (fold change > 1.0) in the PBNP- and FeNP-treated HL60 and KG1a cells and their relationship, ( B ) common DEGs (fold change > 1.5) in a cell treated by two kinds of nanoparticles, ( C ) common DEGs (fold change > 1.5) in two cells treated by a nanoparticle, ( D ) common DEGs (fold change > 1.0) in two cells treated by two kinds of nanoparticles, and ( E ) schematic of cellular functions of common DEGs (fold change > 1.0) in two cells treated by two kinds of nanoparticles. The detailed information of all DEGs is shown in . HF, FeNP-treated HL60 cells; HP, PBNP-treated HL60 cells; KF, FeNP-treated KG1a cells; and KP, PBNP-treated KG1a cells.

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Comparisons of differentially expressed genes (DEGs) in two Leukemia cells treated with two kinds of iron nanoparticles: ( A ) number of upregulated and downregulated DEGs (fold change > 1.0) in the PBNP- and FeNP-treated HL60 and KG1a cells and their relationship, ( B ) common DEGs (fold change > 1.5) in a cell treated by two kinds of nanoparticles, ( C ) common DEGs (fold change > 1.5) in two cells treated by a nanoparticle, ( D ) common DEGs (fold change > 1.0) in two cells treated by two kinds of nanoparticles, and ( E ) schematic of cellular functions of common DEGs (fold change > 1.0) in two cells treated by two kinds of nanoparticles. The detailed information of all DEGs is shown in . HF, FeNP-treated HL60 cells; HP, PBNP-treated HL60 cells; KF, FeNP-treated KG1a cells; and KP, PBNP-treated KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques:

Top 20 GO terms of the KG1a cells treated with two kinds of iron nanoparticles: ( A , B ) heatmap ( A ) and enrichment network ( B ) colored by the same cluster of GO terms in the FeNP-treated KG1a cells, and ( C , D ) heatmap ( C ) and enrichment network ( D ) colored by the same cluster of GO terms in the PBNP-treated KG1a cells. The colored labels followed the order of top 20 GO terms. ( E ) Venn analysis of top 20 GO terms in the KG1a cells treated with PBNPs and FeNPs. The detailed information of all GO terms is shown in .

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Top 20 GO terms of the KG1a cells treated with two kinds of iron nanoparticles: ( A , B ) heatmap ( A ) and enrichment network ( B ) colored by the same cluster of GO terms in the FeNP-treated KG1a cells, and ( C , D ) heatmap ( C ) and enrichment network ( D ) colored by the same cluster of GO terms in the PBNP-treated KG1a cells. The colored labels followed the order of top 20 GO terms. ( E ) Venn analysis of top 20 GO terms in the KG1a cells treated with PBNPs and FeNPs. The detailed information of all GO terms is shown in .

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques:

GO terms of lipid metabolisms in  KG1a  and HL60 exposed to FeNPs or PBNPs (Top 20, p < 0.01).

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: GO terms of lipid metabolisms in KG1a and HL60 exposed to FeNPs or PBNPs (Top 20, p < 0.01).

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Membrane, Activity Assay

GO terms of metal ion metabolisms in  KG1a  and HL60 exposed to FeNPs or PBNPs (Top 20, p < 0.01).

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: GO terms of metal ion metabolisms in KG1a and HL60 exposed to FeNPs or PBNPs (Top 20, p < 0.01).

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Activity Assay, Clinical Proteomics, Membrane

Comparative Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the DEGs ( p < 0.05): ( A ) comparative top 10 enriched pathways of HL60 and KG1a treated with FeNPs and ( B ) comparative top 10 enriched pathways of HL60 and KG1a treated with PBNPs. The pathways in black and blue were HL60 and KG1a cells, respectively. ( C ) Four-way Venn analysis of all KEGG pathways in four groups. The detailed information of all KEGG pathways is shown in . HF, FeNP-treated HL60 cells; HP, PBNP-treated HL60 cells; KF, FeNP-treated KG1a cells; and KP, PBNP-treated KG1a cells.

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Comparative Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the DEGs ( p < 0.05): ( A ) comparative top 10 enriched pathways of HL60 and KG1a treated with FeNPs and ( B ) comparative top 10 enriched pathways of HL60 and KG1a treated with PBNPs. The pathways in black and blue were HL60 and KG1a cells, respectively. ( C ) Four-way Venn analysis of all KEGG pathways in four groups. The detailed information of all KEGG pathways is shown in . HF, FeNP-treated HL60 cells; HP, PBNP-treated HL60 cells; KF, FeNP-treated KG1a cells; and KP, PBNP-treated KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques:

Validation of RNA-Seq DEGs using RT-qPCR: ( A , B ) the expression levels of 10 selected DEGs in the iron nanoparticle-treated HL60 ( A ) and KG1a ( B ) cells. All values are mean ± SD with n = 3. ns, no significance; *, p < 0.05; **, p < 0.01, ***, p < 0.001, ****, and p < 0.0001. ( C , D ) The comparison of fold change detected by RT-qPCR and RNA-Seq in the iron nanoparticle-treated HL60 and ( C , D ) KG1a cells.

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Validation of RNA-Seq DEGs using RT-qPCR: ( A , B ) the expression levels of 10 selected DEGs in the iron nanoparticle-treated HL60 ( A ) and KG1a ( B ) cells. All values are mean ± SD with n = 3. ns, no significance; *, p < 0.05; **, p < 0.01, ***, p < 0.001, ****, and p < 0.0001. ( C , D ) The comparison of fold change detected by RT-qPCR and RNA-Seq in the iron nanoparticle-treated HL60 and ( C , D ) KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Biomarker Discovery, RNA Sequencing, Quantitative RT-PCR, Expressing, Comparison